The wild‐type Schizosaccharomyces pombe mat1 imprint consists of two ribonucleotides

Two-step activation of meiosis by the mat1 locus in Schizosaccharomyces pombe.

The mat1 locus is a key regulator of both conjugation and meiosis in the fission yeast Schizosaccharomyces pombe. Two alternative DNA segments of this locus, mat1-P and mat1-M, specify the haploid cell types (Plus and Minus). Each segment includes two genes: mat1-P includes mat1-Pc and mat1-Pm, while mat1-M includes mat1-Mc and mat1-Mm. The mat1-Pc and mat1-Mc genes are responsible for establis...

The Schizosaccharomyces pombe imprint — nick or ribonucleotide(s)?

(2004). The motion-induced position shift depends on the perceived direction of bistable quartet motion. influence of test patterns on the perception of motion aftereffect and position. (2004). Asymmetry in visual cortical circuits underlying motion-induced perceptual mislocalization. The nature of the Schizosaccharomyces pombe mat1 imprint, which acts to initiate mating-type switching, has bee...

Response to: The Schizosaccharomyces pombe imprint — nick or ribonucleotide(s)?

consistent with a one-ribonucleotide imprint; moreover, the authors do not attempt to detect molecules with a 5′ ′ end corresponding to a one-nucleotide gap. In addition, if the strand were broken due to hydrolysis of an RNA imprint, the 5′ ′ end would be devoid of any ribonucleotide(s); therefore, an RNase T2 control digestion (presented in the paper) is not expected to affect the outcome. Las...

[Schizosaccharomyces pombe].

Cloning of the Schizosaccharomyces pombe

Diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) asymmetric hydrolase (EC 3.6.1.17) is a specific catabolic enzyme of Ap4A found in Schizosaccharomyces pombe. We have previously described the partial purification ofAp4A hydrolase from S. pombe [Robinson, de la Pefia and Barnes (1993) Biochim. Biophys. Acta 1161, 139-148]. We determined the sequence of the N-terminal 20 amino acids of Ap4A hydrola...